Cat. No.
MABL-2456
Application
immunoblot, IP
Isotype
Engineer antibody
Species Reactivity
Saccharomyces cerevisiae, Human, Mouse
Clone No.
17-3-4-1
From
Recombinant Antibody
Specificity
This antibody is specific for N(6)-methyladenosine, a modified base found frequently in the mRNA of all higher eukaryotes and Saccharomyces cerevisiae. It recognises N(6)-methyladenosine 6A in single-stranded nucleic acids, including both DNA and RNA.
Alternative Names
m6A; N6-methyladenosine-5'-mono-phosphate
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with hapten N6-methyladenosine-5’- monophosphate conjugated to BSA
Application Notes
This antibody has been used in immunoprecipitation experiments to identify the presence of N(6)-methyladenosine within mRNA transcripts bound to oligo(dT) magnetic beads (Bodi et al, 2010). It has been characterised using immunoblot analysis (Bodi et al, 2010).
Antibody First Published
Bodi et al. Yeast targets for mRNA methylation. Nucleic Acids Res. 2010 Sep;38(16):5327-35 PMID:20421205
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
