Cat. No.
MABL-2457
Application
inhibition, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NL7
From
Recombinant Antibody
Specificity
The antibody recognises NADPH oxidase 2 (Epitope EKDVITGLK). The antibody cross-reacts with PDIA3.
Alternative Names
NOX2; CYBB; flavocytochrome b; Cyt b; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; gp91phox; p22 phagocyte B-cytochrome
UniProt
P04839
Immunogen
The antibody was generated by immunizing BALB/c mice with Cyt b.
Application Notes
The antibody detected gp91phox by western blot analysis (Burritt et al., 2003; PMID: 12794137 and Bechor et al., 2015; PMID: 25699251). This antibody was used for detection of gp91phox by flow cytometry. The specificity of the antibody for gp91phox was further confirmed by phage display epitope mapping. The antibody inhibited the NADPH oxidase activity before or during the initial stages of electron transfer (Burritt et al., 2003; PMID: 12794137).
Antibody First Published
Burritt et al. Functional epitope on human neutrophil flavocytochrome b558 J Immunol. 2003 Jun 15;170(12):6082-9. PMID:12794137
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
