Cat. No.
MABL-2488
Application
IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Macaque, Human
Clone No.
Bab281
From
Recombinant Antibody
Specificity
The antibody is specific for human NKp46. The antibody cross-reacts with macaque NK46 but does not cross-react with mouse NKp46. NKp46 is a receptor expressed by NK cells.
Alternative Names
Natural cytotoxicity triggering receptor 1; Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; hNKp46; CD335
UniProt
O76036
Immunogen
The original antibody was generated by immunizing Balb/c mice with recombinant human NKp46 protein.
Application Notes
The antibody was used to immunoprecipitate NKp46 molecules from NK and COS-7 cells (Pessino et al., 1998; PMID 9730896 and Cantoni et al. 1999; PMID: 10049942). NK cells were analyzed for NKp46 surface expression by immunofluorescence and Flow Cytometry analysis using the antibody (Sivori et al., 1999; PMID: 10359120). The antibody binding to NKp46 promoted an increase of cytolytic activity in NK cells (Sivori et al., 1997; PMID: 9314561 and Sivori et al., 1999; PMID: 10359120).
Antibody First Published
Sivori et al. p46, a novel natural killer cell-specific surface molecule that mediates cell activation J Exp Med. 1997 Oct 6;186(7):1129-36. PMID:9314561
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
