Cat. No.
MABL-2571
Application
Blocking, functional assays, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PB1.3
From
Recombinant Antibody
Specificity
PB1.3 recognizes human P-selectin. It reacts with human recombinant P-selectin and purified P- selectin, as well as with P-selectin on thrombin-activated platelets. In contrast, it does not cross-react with recombinant E- or L-selectin. P-selectin is a Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes.
Alternative Names
CD62P; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; MAb PB1.3
UniProt
P16109
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing RBF/DnJ mice with human platelets and purified human P-selectin.
Application Notes
Antibody First Published
Mulligan et al. Neutrophil-dependent acute lung injury. Requirement for P- selectin (GMP-140) J Clin Invest. 1992 Oct;90(4):1600-7. doi: 10.1172/JCI116029. PMID:1383277
Note on publication
Describes the generation and characterization of the original mouse IgG1 version of PB1.3.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
