Cat. No.
MABL-2572
Application
WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PL7-6
From
Recombinant Antibody
Specificity
This antibody is specific for human P-selectin, which is expressed by activated platelets.
Alternative Names
GMP-140; SELP; CD62 antigen-like family member P; Granule membrane protein 140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P
UniProt
P16109
Immunogen
This antibody was raised by immunising BALB/c mice with washed platelets.
Application Notes
This antibody has been used in flow cytometric detection of thrombin-stimulated platelets (Katayama et al, 1992), and a chimeric mouse/human version of this antibody also preferentially reacts with activated platelets (Kurome et al, 1994). This could enable the detection of platelets aggregating at sites of blood vessel injury, or the delivery of clot-dissolving drugs. The specificity of this antibody for CD62 has been confirmed in western blot and ELISA (Katayama et al, 1992), and ELISA analysis based on this antibody has been used to assess P-selectin levels in plasma sample of patients post-angina (Ikeda et al, 1995).
Antibody First Published
Katayama et al. A monoclonal antibody-based enzyme immunoassay for human GMP-140/P-selectin Journal of Immunological Methods, Volume 153, Issues 1–2, 30 August 1992, Pages 41-48 PMID:1381405
Note on publication
Describes the original generation of this antibody, and its use in ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
