Cat. No.
MABL-2574
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
pT212/pS214_1
From
Recombinant Antibody
Specificity
This antibody is specific for phosporilated human tau protein.
Alternative Names
ptau; phospho-tau
UniProt
Immunogen
The original antibody was generated by immunizing chickens with phosphorylated tau peptides conjugated to KLH, constructing an scFv phagemid library from spleen and bone marrow RNA, and selecting specific binders through ELISA screening of the phage library against biotinylated phospho- peptides.
Application Notes
The specificity of the original antibody (chicken scFv, lambda) was evaluated via an ELISA. A chimeric human IgG1 version of the antibody was generated. The chimeric variant preserved the original binding characteristics as demonstrated in an ELISA. The binding affinity (KD) of the chimeric antibody was measured at 97.9 nM via SPR (Shih et al., 2012; PMID: 23148212).
Antibody First Published
Shih et al. An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. J Biol Chem. 2012 Dec 28;287(53):44425-34. doi: 10.1074/jbc.M112.415935. Epub 2012 Nov 12. PMID:23148212
Note on publication
The original publication describes the generation and structural characterization of highly specific recombinant chicken antibodies against three phosphorylated tau epitopes associated with Alzheimer's disease.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
