Cat. No.
MABL-2575
Application
IP, WB
Isotype
Engineer antibody
Species Reactivity
Chicken, Hamster, Rat, Human, Mouse
Clone No.
2B12
From
Recombinant Antibody
Specificity
The antibody recognizes mouse, rat, hamster, human and chicken p120/catenin, but it does not cross react with the porcine and canine homologs. The epitope is located at amino acid residues 55‐101.
Alternative Names
Catenin delta-1; Cadherin-associated Src substrate; CAS; p120 catenin; p120(ctn); p120(cas)
UniProt
O60716
Immunogen
The original antibody was generated immunizing A/J mice with tyrosine phosphoproteins purified from chicken embryonic fibroblasts (CEFs) expressing pp60/c-src 527F and 2A/527F.
Application Notes
The antibody (IgG1) was used to immunoprecipitate tyrosine phosphoprotein P120. The antibody was used in Western blot (Kanner at al, 1990; PMID:2110361). The antibody was used to analyse the level of tyrosine phosphorylation of P120 in uninfected CE cells and cells expressing several oncogenic and nononcogenic src variants. The antibody was used to immunoprecipitate P120 from CE cell extracts, then the antibody was used in western blot (Kanner et al, 1991; PMID:1703631). The antibody was employed to perform immunocytochemical analysis in order to investigate the cellular localization of p120 on CMT 93/69 and MDCK cells (Staddon et al, 1995; PMID: 7615637). The antibody immunoprecipitated only CAS1 isoforms (Mo et al., 1996). The antibody was used for immunoprecipitation and Western blotting of pp120 from MDCK cell lysates (Dillon et al, 1998; PMID: 9422525).
Antibody First Published
Kanner et al. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases Proc Natl Acad Sci U S A.1990 May;87(9):3328-32. PMID:2110361
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
