Cat. No.
MABL-2599
Application
ICC, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C-11
From
Recombinant Antibody
Specificity
This antibody is specific for cytokeratin.
Alternative Names
UniProt
Immunogen
The original antibody was raised by immunizing a BALB/c mouse with keratin-enriched preparations from cultured A431 cells.
Application Notes
The original version of this antibody was used for immunocytochemistry on endometrial tissue from whole uteri of regularly cycling women (Ciocca et al., 1983; PMID: 6348061). This antibody was also used for immunohistochemistry on CIS from a DMBA-treated rat (Heffelfinger et al., 2003; PMID: 12861040), on histological sections of CIS from untreated and 6-week ZD6474 cohorts (Heffelfinger et al., 2004; PMID: 15170218), on mouse thymus (Song et al., 2008; PMID: 18824586), and on fresh samples from either tumors or normal tissues distant from tumors (Bártek et al., 1991; PMID: 1716305). This antibody was also used for immunofluorescence on Skin pieces of juvenile goldfish (Mauger et al., 2009; PMID: 19068235). This antibody was used in a western blot on mouse intestine lysates (Rhee et al., 2009; PMID: 19188353), on K562 and MDAMB231 cell lines (Kershaw et al., 2015; PMID: 25957999), and on filament-enriched preparations using this antibody (Bártek et al., 1991; PMID: 1716305).
Antibody First Published
Bártek et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology J Pathol. 1991 Jul;164(3):215-24. PMID:1716305
Note on publication
The original paper describes a series of 14 new mouse monoclonal antibodies (MAbs) to keratins, suggesting their potential value in the differential diagnosis of human tumors.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
