Cat. No.
MABL-2600
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
non-human primates, Human
Clone No.
8F1
From
Recombinant Antibody
Specificity
This antibody reacts with IgG of most primate species, including human IgG.
Alternative Names
UniProt
Immunogen
This antibody was raised by immunising mice with baboon IgG and screening hybridomas against IgG of different primate species.
Application Notes
This antibody has been used in ELISA analysis to quantify IgG in supernatants obtained from cynomolgus macaque lung and lymph node tissue homogenates (Phuah et al, 2012). Additionally, this antibody has been used in a plasma cell ELISPOT assay to quantify the number of IgG-secreting plasma cells in cynomolgus macaque lung and lymph node samples (Phuah et al, 2012). A biotinylated version of this antibody has been used to detect macaque serum IgG bound to high-density peptide microarrays representing different ZIKV polyproteins (Heffron et al, 2018).
Antibody First Published
Phuah et al. Activated B cells in the granulomas of nonhuman primates infected with Mycobacterium tuberculosis. Am J Pathol. 2012 Aug;181(2):508-14. PMID:22721647
Note on publication
This antibody is from the laboratory of Keith Reimann, DVM, Beth Israel Deaconess Medical Center.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
