Cat. No.
MABL-2601
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Pig (Sus scrofa)
Clone No.
AMYL-D10
From
Recombinant Antibody
Specificity
This antibody binds an epitope outside the catalytic site of the porcine pancreatic alpha- amylase.
Alternative Names
AMY2; PA; PPA; 1;4-alpha-D-glucan glucanohydrolase; Porcine pancreatic α-amylase; AMD10; CAB10; cAb‐AMD10
UniProt
P00690
Immunogen
The original antibody was generated by immunizing dromedary with the enzyme and isolating specific binders from the VHH cloned from peripheral blood lymphocytes.
Application Notes
This antibody can be used to detect porcine pancreatic alpha-amylase using and ELISA (PMID: 9649422). The binding of this antibody to alpha-amylase did not inhibit the amylase activity (PMID: 11960990).
Antibody First Published
Lauwereys et al. Potent enzyme inhibitors derived from dromedary heavy- chain antibodies. The EMBO Journal (1998), Vol.17 No.13, pp.3512–3520. PMID:9649422
Note on publication
Describes the generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.