Cat. No.
MABL-2659
Application
Block, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
24F.10C12
From
Recombinant Antibody
Specificity
This antibody binds human PD-L2, expressed on dendritic cells, IFN-gamma-treated monocytes, placental endothelium and medullary thymic epithelial cells.
Alternative Names
CD273; PDL2; PDCD1LG2; PDCD1L2; PD-1 ligand 2; PDCD1 ligand 2; PDCD1 ligand 2; Programmed cell death 1 ligand 2; Butyrophilin B7-DC; B7-DC
UniProt
Q9BQ51
Immunogen
Application Notes
This antibody can recognize PD-L2-Fc in an ELISA. Flow cytometric analysis with whole anti-PD-L2 mAb 24F.10C12 and its Fab version revealed approximately the same mean fluorescence intensity upon binding PD-L2 transfected cells. This antibody is capable of blocking the binding of PD-1-Ig to PD-L2 in a dose-dependent manner and with high avidity. This antibody was also used for the immunohistochemical studies of various tissues including thymus, germinal center, placenta, cardiac tissues, and tumors. Blockade of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production, including that of IFN-gamma and IL-10. Both the whole antibody version and Fab version enhanced T cell activation, showing that PD-L2 functions to inhibit T cell activation (PMID: 12538684). This antibody was used for the study of PD-1 pathway on the activation of naive, memory, and recently activated human CD4+ T cells (PMID: 15598424). Antibody blockade of endothelial cell PD-L2 enhanced endothelial cell costimulation of PHA-activated human CD8+ T cells (PMID: 14579280).
Antibody First Published
Brown et al. Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production. J Immunol. (2003); 170(3):1257-66. PMID:12538684
Note on publication
Describes the generation of this antibody and its tests its capability to block interaction between PD-1 and PD-L2 on dendritic cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
