Cat. No.
MABL-2671
Application
IB, ELISA
Isotype
Engineer antibody
Species Reactivity
Streptococcus pneumoniae (S. Pneumoniae)
Clone No.
E2
From
Recombinant Antibody
Specificity
This antibody is specific for the Streptococcus pneumoniae Pep27. The epitope recognized by this antibody is located within the residues 18-27, with key residues crucial for the binding being Y24, R26, and K27, as identified through alanine scanning and further supported by molecular docking analyses. Streptococcus pneumoniae Pep27 is a small, secreted peptide consisting of 27 amino acids initially described as a "death signal peptide".
Alternative Names
Pep27 protein
UniProt
B2IMR9
Immunogen
The original antibody was selected from a synthetic human scFv library encoding HA-tagged scFv clones through five rounds of biopanning against synthetic and recombinant Pep27.
Application Notes
The dissociation constant (Kd) of the original human scFv version of this antibody was 1.1 µM as measured in an ELISA. This antibody specifically detected Pep27 in an immunoblot and in an environment mimicking in vivo conditions, demonstrated in human serum (Kim et al. 2018; PMID: 29753735).
Antibody First Published
Kim et al., Molecular characterization of single-chain antibody variable fragments (scFv) specific to Pep27 from Streptococcus pneumoniae. Biochem Biophys Res Commun. 2018 Jun 27;501(3):718-723. doi: 10.1016/j.bbrc.2018.05.054. PMID:29753735
Note on publication
The original publication describes the development and characterization of two monoclonal scFvs that are specific to Streptococcus pneumoniae Pep27.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
