Cat. No.
MABL-2749
Application
WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Arabidopsis thaliana
Clone No.
mAbPRF12a (2-11-H7)
From
Recombinant Antibody
Specificity
This antibody recognizes Profilin-1, Profilin-2 and has a weak binding to Profilin-3. Profilins are actin-binding proteins involved in the processing of actin cytoskeleton. They are also recognized allergens.
Alternative Names
Ara t 8; PRO1; Profilin-1; Profilin 1; Profilin; 2-11-H7; PRF1; 2-11H7; Profilin 2; PRO2; Profilin 3
UniProt
Q42449; Q42418
Immunogen
This antibody was raised by immunising mice with a purified plant Profilin-1.
Application Notes
mAbPRF12a antibody is recommended for the analysis and detection of Profilin-1. It strongly identifies Profilin-1 (and Profilin-2) via Western blot (Kandasamy et al., 2002). This antibody was used in immunohistochemical and immunofluorescence analyses to identify parts of the plant where profilins are most prevalent in (Kandasamy et al., 2002).
Antibody First Published
Kandasamy et al. Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton. 2002 May;52(1):22-32. PMID:11977080
Note on publication
This article describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
