Cat. No.
MABL-2755
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
B14+ LysH31-HisH32
From
Recombinant Antibody
Specificity
The antibody binds progesterone and also has less affinity for 5β and 5α-dihydroprogesterone. Progesterone is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species.
Alternative Names
P4; pregn-4-ene-3,20-dione; Agolutin; 17α-progesterone; Corpus luteum hormone
UniProt
Immunogen
This antibody is an affinity matured version of the parent antibody P15G12C12G11 and was isolated from an scFv library generated from wild type scFv P15G12C12G11 using overlap PCR via simultaneous saturation mutagenesis at positions L94, L95, L96 (CDR3L), H50, and H58 (CDR2H).
Application Notes
The binding specificity of this antibody towards progesterone, 5β and 5α- dihydroprogesterone and determination of percentage cross reactivity was done using ELISA. It shows an increase in discrimination against 5β-DHP and 5α-DHP, 23-fold and 15-fold, respectively, compared with the parent antibody (PMID: 15878862).
Antibody First Published
Dubreuil et al. Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineering. J Biol Chem. 2005 Jul 1;280(26):24880-7. PMID:15878862
Note on publication
The specificity of anti-progesterone P15G12C12G11 antibody was improved by combination of in vitro scanning saturation mutagenesis and error-prone PCR.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
