Cat. No.
MABL-2791
Application
IP, WB
Isotype
Engineer antibody
Species Reactivity
Primate, Human
Clone No.
1h3/13
From
Recombinant Antibody
Specificity
This antibody is specific against Rad51B (does not cross-react with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis). Rad51B is a paralog of Rad51 which is an E. coli RecA homologue. It is invloved in the homologous recombination repair (HRR) pathway which fixes double-stranded breaks.
Alternative Names
DNA repair protein RAD51 homolog 2; R51H2; RAD51 homolog B; RAD51- like protein 1; REC2; RAD51L1; 1 H3/13
UniProt
O15315
Immunogen
This antibody was raised against His-tagged recombinant human Rad51B.
Application Notes
WB: rad51b 1h3/13 (1H3) antibody was used to visualise Rad51B on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001).
Antibody First Published
Masson et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes Dev. 2001 Dec 15;15(24):3296-307. PMID:11751635
Note on publication
This article describes the earliest use of rad51b 1h3/13 (1H3) antibody to detect Rad51B protein via Western blotting. However, the exact process of generation of this antibody is described in the following paper (where similar antibodies were generated): Masson, J. Y., Stasiak, A. Z., Stasiak, A., Benson, F. E., & West, S. C. (2001). Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proceedings of the National Academy of Sciences, 98(15), 8440-8446.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
