Cat. No.
MABL-2954
Application
Blocking, crystallisation, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
5E 1
From
Recombinant Antibody
Specificity
This antibody is specific for the amino-terminal domain of rat SHH (SHH-N). It cross-reacts with the two other mammalian hedgehog ligands, Indian Hedgehog and Desert Hedgehog.
Alternative Names
shh; sonic hh; Q15465
UniProt
Q63673
Immunogen
This antibody was raised by immunizing mice with purified rat SHH amino-terminal domain protein.
Application Notes
The binding specificity of this antibody for the amino-terminal fragment of SHH (SHH- N) has been confirmed in ELISA and Western blot analysis (Ericson et al, 1996).This antibody has been used to detect SHH in IHC analysis (Ericson et al, 1996).This antibody is able to inhibit hedgehog signalling, as it blocks the binding of all three mammalian Hh ligands to Ptc1 with low nanomolar affinity (Bosanac et al, 2009). In vivo, this inhibition is responsible for the observed growth retardation of HT55 an HT-29 xenografts in mice tumour models (Yauch et al, 2008). A Fab fragment derived from this antibody has been used in X ray crystallography analysis, in complex with human SHH (Maun, 2010).
Antibody First Published
Ericson et al. Two Critical Periods of Sonic Hedgehog Signaling Required for the Specification of Motor Neuron Identity Cell, Volume 87, Issue 4, 15 November 1996, Pages 661-673 PMID:8929535
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA, WB and IHC analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
