Cat. No.
MABL-3026
Application
bioluminescent enzyme immunoassay, chemiluminescent enzyme immunoassay, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
3F9
From
Recombinant Antibody
Specificity
This antibody is specific for tenuazonic acid, an Alternaria mycotoxin. The antibody does not cross-react with AOH, AME, DON, ITeA, or ZEN.
Alternative Names
Nb-3F9; TeA; Alternaria mycotoxin tenuazonic acid; (5S)-3-Acetyl-5-[(2S)- butan-2-yl]-4-hydroxy-1,5-dihydro-2H-pyrrol-2-one
UniProt
Immunogen
The original antibody was generated by immunizing a male Bactrian camel with TeA-CMO- KLH followed by antibody library construction and phage display-based selection.
Application Notes
The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6×His tag secondary antibody. The IC50 value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.
Antibody First Published
Wang et al. Chemiluminescent Enzyme Immunoassay and Bioluminescent Enzyme Immunoassay for Tenuazonic Acid Mycotoxin by Exploitation of Nanobody and Nanobody- Nanoluciferase Fusion Anal Chem. 2020 Sep 1;92(17):11935-11942. doi:32702970 10.1021/acs.analchem.0c02338 PMID:
Note on publication
The original publication describes the development of highly specific nanobodies (Nbs), particularly Nb-3F9, against the Alternaria mycotoxin tenuazonic acid (TeA) using a stringent biopanning strategy.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
