Cat. No.
MABL-3050
Application
functional assay, IHC-P, IP, neutralize, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Cow, Dog, Hamster, Monkey, Rat, Human, Mouse
Clone No.
1D11.16.8
From
Recombinant Antibody
Specificity
This antibody recognizes and binds human, mouse, rat, cow, dog, hamster, monkey and other non-human primate transforming growth factor (TGF) beta 1, 2 and 3. TGF beta is a multifunctional cytokine that exists as five isoforms (TGF beta 1-5) that share 70-80% homology with each other. These proteins are produced by many cell types and function as growth and differentiation factors.
Alternative Names
TGF-β; TGFβ; TGFB1; TGFB2; TGFB3; TGF-B1; TGF-B2; TGF-B3; TGF beta 1; TGF beta 2; TGF beta 3; CED; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; transforming growth factor; transforming growth factor beta; 1D11.16; 1D11
UniProt
P18341; P21214; A6QP91
Immunogen
The original antibody was generated by intraperitoneally (IP) injecting female Balb/c mice with 10 ug of native bovine TGF-B2 with complete Freund's adjuvant.
Application Notes
This antibody was able to neutralize the biological activity of both TGF-beta 1 and beta 2 in vitro. This antibody could detect the dimeric form of TGF-beta 2 in western blots. This antibody was also reported to immunoprecipitate [125I]-TGF-beta 2 (PMID: 2537357). This antibody is also able to neutralize the growth promoting actions of TGF-B1 and B2 in the NRK proliferation assay and TGF-B induced alkaline phosphatase production in an assay using the ROS 17.2.8 osteosarcoma cell line (US5571714). This antibody was used for detection of TGF-β production in vivo in spleens sections of L. donovani-infected hamsters using immunohistochemistry (PMID: 9488418). This antibody was also used for the immunohistochemical analysis of FFPE human renal biopsy specimens for presence of TGF-beta (PMID: 10515378).
Antibody First Published
Dasch et al. Monoclonal antibodies recognizing transforming growth factor- beta. Bioactivity neutralization and transforming growth factor beta 2 affinity purification. J Immunol. 1989 Mar 1;142(5):1536-41. PMID:2537357
Note on publication
Describes the generation and characterization of 4 antibodies against TGF beta.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
