Cat. No.
MABL-3081
Application
Inhibiting, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RMT1-10
From
Recombinant Antibody
Specificity
This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. IgV domain of Tim-1 was proposed as the epitope of RMT1-10 (Xiao et al., 2007; pmid: 17606630). RMT1-10 did not corss-react with Tim-4-Ig fusion proteins. 3B3 - an anti-Tim-1 antibody with opposite, T-cell activatory effects blocked binding of RMT1-10 suggesting the same or very similar epitope (Xiao et al., 2007; pmid: 17606630). Different functional activity between RMT1-10 and 3B3 might stem from the fact that they exhibited different binding avidity to Tim-1 (Xiao et al., 2007; pmid: 17606630). TIM-1 has been found on CD4+ T cells, mast cells, and a subset of B cells. TIM-1 is involved in renal injury, tissue homeostasis, and T cell costimulation (positive and negative). TIM-1 can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself.
Alternative Names
CD365; HAVcr-1; HAVCR1; Hepatitis A virus cellular receptor 1 homolog; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; T cell membrane protein 1; T-cell immunoglobulin and mucin domain containing 1; T-cell immunoglobulin mucin receptor 1; TIM-1; TIM1; TIMD-1
UniProt
Q5QNS5
Immunogen
The original rat IgG2a version of this antibody was raised by immunizing SD rats with full- length Tim-1-Ig that contained both IgV and mucin domains of Tim-1. Lymph node cells were then fused with P3U1 myeloma cells and cloned creating hybridomas.
Application Notes
This anti-Tim-1 antibody inhibits the generation of antigen-specific T cells, production of IFN-γ and IL-17, and development of autoimmunity, and it caused a strong Th2 response (Xiao et al., 2007; pmid: 17606630). RMT1-10 gives then rise to opposite effects than those brought about by another anti-Tim1 clone - 3B3 (Ab01032) which achtivates T-cells. The use of RMT1-10 demonstrated that binding Tim-1 can not only lead to activation of T-cells (as it had been known before) but that it can also cause the aforementioned inhibition (Xiao et al., 2007; pmid: 17606630). Consequently, the opposite antibodies RMT1- 10 and 3B3 (Ab01032) can be utilized together to study various aspects of Tim-1 costimulation and its consequences. This antibody was used in multiple flow cytometry (FC/FACS) experimetns, such as identifying Tim-1-expressing EL-4 cells to determine the antibody's epitope (Xiao et al., 2007; pmid: 17606630).
Antibody First Published
Xiao et al. Differential engagement of Tim-1 during activation can positively or negatively costimulate T cell expansion and effector function J Exp Med. 2007 Jul 9;204(7):1691-702. doi: 10.1084/jem.20062498. Epub 2007 Jul 2. PMID:17606630
Note on publication
Deescribes the generation and characterization of the antibody RMT1-10.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
