Cat. No.
MABL-3091
Application
FACS, in vivo, IP, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
5G3
From
Recombinant Antibody
Specificity
This antibody is specific for murine TIM-4. TIM-4, unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen-presenting cells such as lymphoid and myeloid dendritic cells and macrophages. TIM-4 has been shown to interact with TIM-1, thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally, TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in the engulfment of apoptotic cells.
Alternative Names
TIMD4; SMUCKLER; SMUCKLER, TIM4; Spleen, mucin-containing, knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4
UniProt
Q6U7R4
Immunogen
This antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein.
Application Notes
The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays. The antibody was also used to immunoprecipitate Tim-4 protein from lysates of CHO-Tim-4 as part of WB assays (Rodriguez-Manzanet et al., 2008; PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b+ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010; PMID: 20368430). treatment with this antibody alone did not significantly affect tumor burden or survival, but it enhanced the protective effect of anti-PD-1 blockade. The combined treatment resulted in an increase in total CD8+ and CD8+ CD39 + T cells in the peritoneal cavity. RNA sequencing of sorted Tim-4+ macrophages revealed genes differentially expressed between animals treated with anti-PD-1 alone and those treated with both anti-PD- 1 and this antibody, indicating that it alters the gene expression profile of Tim-4+ macrophages. Notably, it reduced the expression of genes associated with immunosuppression (Ptgs2, Tgfb2, and Tdo2) while increasing the expression of genes associated with macrophage activation (Stab1 and Mrc1). This antibody was used as part of single-cell RNA sequencing and T-cell receptor sequencing analyses. + T cells (Chow et al., 2021; PMID: 34115989).
Antibody First Published
Rodriguez-Manzanet et al., TIM-4 expressed on APCs induces T cell expansion and survival J Immunol. 2008 Apr 1;180(7):4706-13. PMID:18354194
Note on publication
The original publication characterizes monoclonal antibodies generated to study how TIM-4 induces T-cell expansion and survival.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
