Cat. No.
MABL-3118
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2TY
From
Recombinant Antibody
Specificity
This scFv is specific for human TNF-α.
Alternative Names
TNFSF2; tumor necrosis factor alpha; TNF; C1K3N5; TNFA; Cachectin; TNF-alpha; TNF-a; Tumor necrosis factor ligand superfamily member 2; TNF-α
UniProt
P01375
Immunogen
Affinity selection procedures were used to isolate this scFv from a naïve Tomlinson I phage display library that specifically binds to TNF-α. The panning procedure was carried out by incubating immunotubes coated with recombinant TNF-α protein with a phage suspension. Phage particles with affinity for recombinant TNF-α were amplified in TG-1 bacteria, and three rounds of panning were performed to select high affinity and specificity scFvs.
Application Notes
The binding affinity of this scFv for human TNF-α has been confirmed in ELISA analysis (Safarpour et al, 2018). This scFv could have future therapeutic applications as a TNF inhibitor. TNF inhibition is a strategy currently showing promise in the treatment of a range of inflammatory diseases, including rheumatoid arthritis and Chron's disease.
Antibody First Published
Safarpour et al. Isolation and Characterization of Novel Phage Displayed scFv Fragment for Human Tumor Necrosis Factor Alpha and Molecular Docking Analysis of Their Interactions Iran J Pharm Res. 2018 Spring; 17(2): 743–752. PMID:29881431
Note on publication
Describes the original generation of this scFv by phage display.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
