Cat. No.
MABL-3131
Application
Depletion, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RI7 217.1.3
From
Recombinant Antibody
Specificity
The transferrin receptor is necessary for cellular iron uptake by the process of receptor- mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.
Alternative Names
CD71; Transferrin receptor protein 1; TR; ; TfR1; Trfr; R17 217.1.3; RI7- 217
UniProt
Q62351
Immunogen
The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
Application Notes
This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984; PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989; PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000; PMID: 10688622). Injection of this antibody into mice resulted in CD71+ erythroid splenocyte cell depletion (Torow et al., 2015; PMID: 26195040).
Antibody First Published
Lesley et al, 1984. Expression of transferrin receptor on murine hematopoietic progenitors. Cell Immunol. 1984 Jan;83(1):14-25. PMID:6319033
Note on publication
Describes the original generation of the antibody and its application to flow cytometry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
