Cat. No.
MABL-3151
Application
IP, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Cat, Dog, Horse, Sheep
Clone No.
14H9.E4.A4
From
Recombinant Antibody
Specificity
This antibody recognises ovine, canine, feline and equine TSH beta which is a crucial hormone controlling thyroid functions.
Alternative Names
Thyroid stimulating hormone b; Thyrotropin subunit beta; TSHB; Thyroid- stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain; 14H9.E2
UniProt
P54828
Immunogen
The gene encoding the mature β subunit of canine thyroid stimulating hormone (cTSHβ) was cloned, sequenced and expressed in Escherichia coli and in Chinese hamster ovary (CHO) cells, and monoclonal antibodies against the recombinant cTSHβ, purified from E. coli, were generated.
Application Notes
This antibody is capable of detecting TSH beta in a couple of important veterinary species. It may be used in the identification and analysis of TSH as well as TSH tests in veterinary medicine and research (Ferguson, 2007). For instance, 14H9.E4 linked to agarose beads was used to putify heterodimeric feline TSH (Rayalam et al., 2006).
Antibody First Published
Rayalam et al. Expression and purification of feline thyrotropin (fTSH): immunological detection and bioactivity of heterodimeric and yoked glycoproteins. Domest Anim Endocrinol. 2006 Mar;30(3):185-202. Epub 2005 Aug 10. PMID:16125358
Note on publication
This article describes the original application of this antibody in an immunopurification procedure.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
