Cat. No.
MABL-3189
Application
in vitro, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2H1
From
Recombinant Antibody
Specificity
This amtibody is specific for the human VEGF protein.
Alternative Names
Vascular endothelial growth factor
UniProt
Q9UNS8
Immunogen
The original antibody was raised by panning VEGF against a recombinant human antibody library containing 4.25 × 108 scFvs from non-immunized healthy donors^.
Application Notes
The original antibody was used for surface plasmon resonance on VEGF. This showed the binding affinity of the antibody to its antigen. This antibody was tested in vitro. It was assessed in a VEGF-stimulated HUVEC mitogenesis, it showed it was able to inhibit HUVEC proliferation in a dose dependent matter. This antibody was used for inhibition of VEGF-KDR binding. It showed that it was able to inhibit the binding. This antibody was used for an ELISA on different peptides of its antigen to determine its epitope (Lamdan et al., 2011; PMID:21167224, Lamdan et al., 2013; PMID:23702826).
Antibody First Published
Lamdan et al. Isolation of a novel neutralizing antibody fragment against human vascular endothelial growth factor from a phage-displayed human antibody repertoire using an epitope disturbing strategy J Biotechnol. 2011 Jan 20;151(2):166-74. PMID:21167224
Note on publication
The oriused a human VEGF variant containing three mutations in the region recognized by Bevacizumab to direct antibody selection towards recognition of other epitopes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
