Cat. No.
MABL-3201
Application
Blocking, in vivo, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B1C4A7
From
Recombinant Antibody
Specificity
The antibody is specific for VEGFR2.
Alternative Names
flk-1;FLK-1; KDR; Fetal liver kinase 1; Kinase insert domain receptor; Protein-tyrosine kinase receptor; EC:2.7.10.1; Vascular endothelial growth factor receptor 2; Vascular endothelial growth factor receptor
UniProt
P35968
Immunogen
The original antibody was isolated by panning a phage display library against VEGFR2.
Application Notes
The antibody, together with dsD7A8, was used for constructing the bispecific antibody (A7-A8)-LALA. The original and the bispecific antibody could strongly bind to the recombinant human VEGFR2 and mouse VEGFR2 (EC50 =0.107 nM and 0.466 nM for B1C4A7 and 0.128 nM and 0.598 nM for A7- A8 respectively). The bispecific antibody could strongly block ligand VEGF binding to its receptor VEGFR2 (IC50 for hVEGF-hVEGFR2 interaction was 1.29 nM for the bispecific antibody and 1.72 nM for B1C4A7 and IC50 for mVEGF-mVEGFR2 interaction was 0.801 nM for the bispecific antibody and 0.973 nM for B1C4A7). The original and bispecific antibodies could bind to KDR/PAE cell expressed human VEGFR2, mouse VEGFR2 (EC50 =0.522 nM and 0.533 nM, respectively). The bispecific antibody associated to VEGFR2 fast and dissociated slowly (KD = 47 pM for the bispecific and 752 pM for the original antibody).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
