Cat. No.
MABL-3212
Application
ICC, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Caprine, Hamster, Rat, Swine, Human
Clone No.
RV203
From
Recombinant Antibody
Specificity
The antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Alternative Names
UniProt
P08670
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with a cytoskeletal vimentin extract of calf lens.
Application Notes
The antibody detected vimentin on cytoskeletal extracts of bovine lens, BHK-21 and HeLa cells by western blot analysis. The antibody detected vimentin on connective tissue fibroblasts of hamster tongue, polygonal BHK-21/C13 cells and elongated BHK-21 cells by immunohistochemistry (Schaart et al., 1991; PMID: 2065865).
Antibody First Published
Schaart et al. Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins Differentiation. 1991 Mar;46(2):105-15. PMID:2065865
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
