Key features and details | |
Cat. No. | MABL-1707 |
Name | Anti-Glucose-dependent insulinotropic Receptor mAbs |
Clone No. | AFD- Gipg013 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ELISA, IF |
Species Reactivity | Dog, Rat, Human, Mouse |
Basic Information | |
Specificity | The original has a Ki value of 7 nM and a Kd value of 6.8 nM for human GIPr. |
Alternative Name | GIPr |
UniProt | Q8TDV5 |
Immunogen | Human GIPr. |
Application Notes | This antibody binds to GIPr, a GPCR expressed on pancreatic beta-cells, where activation leads to the release of insulin. Endogeonous ligands for the receptor include oleylethanolamide and lysophosphatidylcholine. The antibody acts as a competitive antagonist. In rat islets, the antibody inhibits GIP-induced secretion of insulin by up to 81%. |
Antibody First Published | Ravn et al. Structural and pharmacological characterization of novel potent and selective monoclonal antibody antagonists of glucose-dependent insulinotropic polypeptide receptor. Journal of Biological Chemistry 2013; 288(27)：19760-19772 PMID：23689510 |
Note on publication | Describes the generation of a monoclonal antibody against GIP and subsequent crystallisation as well as inhibition studies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
