Anti-SARS-CoV S glycoprotein mabs [1B5](MABL-3577)

Cat. No.

MABL-3577

Name

Anti-SARS-CoV S glycoprotein mabs [1B5](MABL-3577)

Clone No.

1B5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

NTRL, ELISA

Species Reactivity

SARSCoronavirus

Basic Information

Specificity

This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).

Alternative Name

Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1

UniProt

P59594

Immunogen

The original antibody was generated by immunizing a non human transgenic animal XENOMOUSE® IgG2k mice against S1 protein-Ig   fragments (ectodomain) of the S Protein (Tor2).

Application Notes

This antibody inhibits the association of SARS-CoV s protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the   virus. The originally characterized antibody had a neutralizing titer   200TCID50 of 12.5 μg/ml (Coughlin et at.,2006) and IC50 of 2.84 μg/ml when   incubated with S12–510- Fc recombinant protein (Coughlin et at.,2009). Purified 1B5 binds to RBD region with a unique CDR3 sequence. When used in   combination with 4D4 the lack of synergistic effect indicated that the mAbs   4D4 and 1B5 likely interact with closely positioned conformational epitopes   and compete with each other for binding (Coughlin et at.,2009). ELISA.

Antibody First   Published

Coughlin et al. Generation and   characterization of human monoclonal neutralizing antibodies with distinct   binding and sequence features against SARS coronavirus using XenoMouse® Virology 361 (2007) 93–102. PMID:17161858

Note on publication

Describes the generation and characterization of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.