Anti-SARS-CoV S glycoprotein mabs [4D4](MABL-3578)

Cat. No.

MABL-3578

Name

Anti-SARS-CoV S glycoprotein mabs [4D4](MABL-3578)

Clone No.

4D4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

NTRL, ELISA

Species Reactivity

SARSCoronavirus

Basic Information

Specificity

This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).

Alternative Name

Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1

UniProt

P59594

Immunogen

The original antibody was generated by immunizing a non human transgenic animal XENOMOUSE® IgG2k mice against S1 protein-Ig   fragments (ectodomain) of the S Protein (Tor2).

Application Notes

This antibody inhibits the association of   SARS-CoV s protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the   virus. The originally characterized antibody had a neutralizing titer   200TCID50 of 12.5 μg/ml (Coughlin et at.,2006). When incubated with S12–510-   Fc recombinant protein it failed to block the interaction of this protein   with the cellular receptor but inhibited a post-binding event and prevented   viral entry. Combination of this antibody with another antibody 5D3, that   binds to a different epitope resulted in increased inhibition of virus. The   ability of mAb 4D4 to more efficiently inhibit viral entry when added post   virus binding suggests that the S protein might undergo a conformational   change following binding to ACE2 leading to unmasking of the epitope   recognized by the 4D4 antibody. Simultaneously, 4D4 was also used in a   standardentry inhibition assay as a positive control for determining the   degree of inhibition of pseudotyped virus entry. (Coughlin et at.,2009). 

Antibody First   Published

Coughlin et al. Generation and characterization of human monoclonal neutralizing antibodies with distinct   binding and sequence features against SARS coronavirus using XenoMouse® Virology 361 (2007) 93–102. PMID:17161858

Note on publication

Describes the generation and characterization of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.