Cat. No.
MAK-0079
Product Name
Mouse IgA
Storage
4°C
Species
Mouse
Instructions
The assay detects IgM from species including Baboon, Rhesus, Cynomolgus and Squirrel monkeys.
Introduction
The mouse IgA ELISA kit is designed for measurement of IgA in serum, plasma, and other fluids. The assay uses goat anti-mouse IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-mouse IgA antibodies for detection. Both capture and detection antibodies were cross absorbed on mouse IgG and IgM agarose columns, thereby ensuring specificity for IgA. Cross-reactivity with immunoglobulins from other species has not been investigated.
Serum IgA levels in the range of 50 – 300 µg/ml have been reported in mice.1,2 Similar levels are found in mouse milk.3 IgA levels increase during malnutrition.2 This kit recognizes monomeric IgA found in serum and the secreted J-chain linked dimer found in milk.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside mouse IgA standards. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. IgA molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgA is proportional to the optical density of the test sample and is derived from a standard curve.
