Cat. No.
MAK-0080
Product Name
Mouse IgE
Storage
4°C
Species
Mouse
Instructions
The mouse IgE ELISA kit designed for measurement of IgE in serum and plasma.
Introduction
IgE is the least abundant immunoglobulin in mice. Basal levels range from 0.1 – 1 µg/ml, depending on mouse strain, but increase several fold during an immune response. IgE is therefore a widely used biomarker in mouse models of allergy, infection and asthma.
Principle Of The Assay
The assay uses goat anti-mouse IgE for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rat anti-mouse IgE antibodies for detection. Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared mouse IgE standards. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. IgE molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgE is proportional to the optical density of the test sample and is derived from a standard curve.
