Cat. No.
MABL-120
Application
ICC, WB, IF
Isotype
Engineer antibody
Species Reactivity
pan, Rat, Human, Mouse
Clone No.
N23B/49
From
Recombinant Antibody
Specificity
This antibody is specific for shank1, shank2 and shank3. Shank2 seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Alternative Names
N23B/49R; SH3 and multiple ankyrin repeat domains protein 2; Cortactin- binding protein 1; CortBP1; GKAP/SAPAP-interacting protein; Proline-rich synapse-associated protein 1; ProSAP1; SPANK-3
UniProt
Q9QX74
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 84- 309 (SH3/PDZ domains) of rat Shank2.
Application Notes
This antibody was used for immunocytochemistry on the hippocampal cultures of rats and mice (MacGillavry et al, 2016; PMID:26547831) (Tao-Cheng et al, 2010; PMID:20347015) (Suh et al, 2010; PMID:20118925). Immunofluorescense was preformed on hippocampal neuronal cells of mice using this anitbody (Pham et al, 2010; PMID:20573181) (Ricciardi et al, 2012; PMID:22922712). This antibody was used to preform a western blot on human brain samples (Pham et al, 2010; PMID:20573181).
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
