Key features and details | |
Cat. No. | MABL-3156 |
Name | Anti-tumor-associated gangliosides mAbs |
Clone No. | AFD- PolrecCHOIgM/GD3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, in vitro, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human GD3, GM1, GD2, and GM3 gangliosides. |
Alternative Name | GD3; GM3; GD2; GM1 |
UniProt | |
Immunogen | The original antibody was developed by isolating heavy and light chain cDNAs from a hybridoma producing an anti-GD3 IgM antibody. These sequences were then codon-optimized and transfected into CHO cells to produce the recombinant PolrecCHOIgM/GD3 antibody. |
Application Notes | The original antibody (recombinant human IgM) was designed to bind the ganglioside GD3, which is overexpressed in malignant cells like melanomas. ELISA testing revealed strong binding specificity to GD3, GM1, GD2, and GM3 gangliosides, even at low concentrations. In cell-line assays, it selectively bound cancer cell lines (melanoma, lung, breast, and glioblastoma), without binding to normal cells such as huVEC and lung fibroblasts. Immunoimaging confirmed this selective binding, with fluorescence indicating strong interaction with malignant cells. In vitro complement activation tests showed that the antibody triggered cancer cell lysis when exposed to normal human serum, demonstrating its ability to activate the complement cascade. The antibody thus effectively discriminates between malignant and normal cells, confirming its diagnostic and therapeutic utility in cancer (US10590204B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
