Key features and details | |
Cat. No. | MABL-3190 |
Name | Anti-VEGF mAbs |
Clone No. | AFD- A.4.6.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, Block, ELISA, FC, IHC |
Species Reactivity | Rat, Human |
Basic Information | |
Specificity | Binds native and reduced human VEGF (isoform 165), with a Kd of 0.8 nm determined for the original clone. |
Alternative Name | VEGF-A; Vascular Endothelial Growth Factor; VEGFA; Vascular Permeability Factor; VPF |
UniProt | P15692 |
Immunogen | Recombinant human VEGF. |
Application Notes | This is the parental antibody clone to the humanized, therapeutic antibody Bevacizumab (marketed as Avastin) binding to VEGF. VEGF is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. |
Antibody First Published | Kim et al. 1992 The Vascular Endothelial Growth Factor Proteins: Identification of biologically relelvant regions by neutralizing monoclonal antibodies. Growth Factors. 1992;7(1):53-64. PMID:1380254 |
Note on publication | Describes generation of anti-hVEGF monoclonal antibodies as well as characterisation of binding by various methods. Also shows inhibition of angiogenesis and vascular permeabilization induced by human VEGF in a model organisms. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
