Cat. No.
MABL-613
Application
functional assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Haemophilus influenzae
Clone No.
CA4
From
Recombinant Antibody
Specificity
This antibody is specific for the capsular polysaccharide protien of Haemophilus influenzae. This protein is part of the polysaccharide layer that lies outside the cell envelope. Haemophilus influenzae causes bacteremia, pneumonia, epiglottitis and acute bacterial meningitis.
Alternative Names
Hib PS
UniProt
Immunogen
This antibody was raised by immunizing a 43-year-old white male using a subcutaneous injection of 40 mg of Hib PS vaccine. Five days later, mononuclear cells (MNC) were isolated from 60 ml of peripheral blood with Lymphoprep. Two cell populations were used for RNA isolation
Application Notes
An ELISA was performed on h Hib PS oligomers coupled to human serum albumin using the human version of this antibody (Reason, Wagner and Lucas, 1997; pmid:8975921). The human version of this antibody was used for an ELISA on Hib PS-poly-L-lysine. Furthermore, an infant rat assay was used to evaluate in vivo protection of this antibody, Five- to 7-day-old rats received a subcutaneous injection of either purified IgGl or IgG2 anti-Hib PS antibodies, purified MAb CA4, or, as a negative control, a human IgG2 myeloma protein. Eighteen to 24 h later, blood was taken and assayed quantitatively on chocolate agar for the presence of Hib organisms. Additionally, this antibody was evaluated by an ELISA for rheumatoid factor activity and for binding to histones, chromatin, and denatured DNA (Lucas, Larrick and Reason, 1994; pmid:8063404).
Antibody First Published
Lucas, Larrick and Reason Variable region sequences of a protective human monoclonal antibody specific for the Haemophilus influenzae type b capsular polysaccharide. Infect Immun. 1994 Sep;62(9):3873-80. PMID:8063404
Note on publication
A hybridoma secreting a human immunoglobulin G2 kappa monoclonal antibody (MAb) specific for the capsular polysaccharide of Haemophilus influenzae type b (Hib) was isolated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
