Cat. No.
MABL-617
Application
in vivo, inhibition, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
G10
From
Recombinant Antibody
Specificity
This antibody is specific for human CA IX.
Alternative Names
CA9; Carbonic anhydrase 9; Carbonate dehydratase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 MN/CA IX; endogenous protein p54/58N; EC
UniProt
Q16790
Immunogen
The original antibody was generated by panning a non-immune human scFv-phage display library against CA IX-expressing cells (293T-G250 cells).
Application Notes
The specificity of this antibody's original format (human scFv) was validated via ELISA, demonstrating its selective binding to CA IX-expressing cells. It was further confirmed through flow cytometry and cross-competition studies. This antibody exhibited an IC50 of 2.086 µg/ml / 2.9 nM to stable G250-expressing 293T cells. This antibody was tested in vivo in Ncr Nude mice (athymic mice); it was incorporated into lentiviral vectors to create anti-CA IX chimeric immune receptors for T-lymphocyte transduction, resulting in enhanced T-lymphocyte cytotoxicity against renal cell carcinoma (RCC) cells. An scFv-Fc fusion version of this antibody was successfully used in an internalization study of anti-CA IX scFv- Fc antibody fusion proteins into stable G250-293T cells, exhibiting mild (~20%) inhibition (US8466263B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
