Cat. No.
MABL-842
Application
ICC, IP, RIA, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-61
From
Recombinant Antibody
Specificity
OX-61 binds specifically to DPP4. Evidence： tissue distribution of OX-61 and DPP4 are identical; equal MWTs; OX-61-mediated reduction in DPP4 activity from solubilized liver homogenates. DPP4 is a glycoprotein receptor which acts as a positive regulator of T cell co-activation.
Alternative Names
DPP4; DPP 4; DPPIV; DPP IV; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; GP110 glycoprotein; T-cell activation antigen CD26 ;OX61; MRC OX-61; MRC OX61
UniProt
P14740
Immunogen
OX-61 was prepared by immunizing mice with rat thoracic duct lymphoid cells that were enriched for dendritic cells.
Application Notes
This antibody can be used for denaturing and non-denaturing WB and developed using peroxidase-conjugated rabbit anti-mouse. It can also be used for IHC of cryostat sections from adult rat liver. Staining is also observed on the brush border of the small intestine and colonic epithelium, T cell areas of lymphoid cells but not B cell follicles
Antibody First Published
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol 24：1281 1987
Note on publication
Describes the use of Ox-48 among other anitbodies to identify new markers for activated T-cells, which might have expression related to theactive Vs quiescient state or may be involved in specific T-cell functions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
