Cat. No.
MABL-992
Application
FACS, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PD7/26
From
Recombinant Antibody
Specificity
This antibody recognises CD45RB which is a transmembrane tyrosine phosphatase that is present on all leukocytes and regulates the threshold of T cell antigen receptor signaling. There are multiple CD45R isoforms resulting from the alternative splicing of this gene. PD7/26 antibody binds to the exon 5.
Alternative Names
Receptor-type tyrosine-protein phosphatase C; PTPRC; Leukocyte common antigen; L-CA; T200; CD45; CD45R; LY5; T200 glycoprotein; T200 leukocyte common antigen
UniProt
P08575
Immunogen
This antibody was raised by immunising Balb/c mice with cells from a human peripheral- blood lymphocyte line maintained in T-cell growth factor.
Application Notes
This antibody has been widely used in the identification of leukocytes (as CD45 is a pan-leukocyte marker) and lymphatic tumours in paraffin-embedded tissue sections. For instance, one group utilised it during the morphological characterisation of patient-derived xenografts via immunohistochemical staining of formalin-fixed, paraffin-embedded samples (Chijiwa et al., 2015). Another group used this antibody for immunofluorescence staining of similarly prepared fibroblast samples in chronic obstructive pulmonary disease (Brand et al., 2015). Finally, Deckert-Schlüter and colleagues (1998) used PD7/26 antibody when researching primary CNS lymphomas.
Antibody First Published
Warnke et al. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med. 1983 Nov 24;309(21):1275-81. PMID:6355845
Note on publication
This article describes generation and characterisation of PD7/26 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
