Cat. No.
MABL-1006
Application
functional inhibition, IP, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C5D5
From
Recombinant Antibody
Specificity
This antibody recongises human CD47 extracellular domain. CD47 is a a multitransmembrane glycoprotein widely expressed on all hematopoietic cells and most other cell types. It is involved in cell adhesion, Ca2+ influx, platelet activation and peripheral mononuclear neutrophils migration across endothelial and epithelial monolayers.
Alternative Names
C5/D5; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6
UniProt
Q08722
Immunogen
This antibody was raised by immunising Balb/c mice with T84 epithelial membranes.
Application Notes
C5/D5 is recommended for staining cell surface CD47 as well as neutralising CD47. Furthermore, it is capable of inhibiting peripheral mononuclear neutrophils migration across polarised monolayers of the human intestinal epithelial cell line T84 in a bidirectional fashion (Parkos et al., 1996). This antibody is also very effective in detecting CD47 in flow cytometry; this was demonstrated with various cells, such as CaCO2 and polymorphonuclear neutrophils (Liu et al., 2001). Finally, C5D5 was also utilised in the immunofluorescence staining of the basolateral surfaces of methanol-fixed CaCO2 monolayers expressing exogenously transfected human CD47 (Liu et al., 2001).
Antibody First Published
Parkos et al. CD47 mediates post-adhesive events required for neutrophil migration across polarized intestinal epithelia. J Cell Biol. 1996 Feb;132(3):437-50. PMID:8636220
Note on publication
This article describes the generation and characterisation of C5D5 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
