Cat. No.
MABL-1039
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LU30
From
Recombinant Antibody
Specificity
The antibody binds to CD55 with a Kd of 13 nM.
Alternative Names
DAF; Complement decay-accelerating factor
UniProt
P08174
Immunogen
Human CD55.
Application Notes
The antibody binds specifically to CD55, a glycophosphatidylinositol-anchored protein which acts together with CD46 and CD59 to protect cells from complement-mediated cell lysis. The antigen recognises C4b, C3 and C3b complement components, and, by blocking their catalytic activity, inhibits the formation of the membrane attack complex. CD55 is overexpressed in many tumour cell lines, thereby inhibiting complement-mediated cell lysis and increases tumour resistance to immune attacks.
Antibody First Published
Ridgway,J.B., Ng,E., Kern,J.A., Lee,J., Brush,J., Goddard,A. and Carter,P. Identification of a human anti-CD55 single-chain Fv by subtractive panning of a phage library using tumor and nontumor cell lines. Cancer Res. 59 (11), 2718-2723 (1999) PMID:10363997
Note on publication
Describes the use of a human single-chain Fv phage library to search tumor- associated antigens and the production of monoclonal antibodies against the antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
