Cat. No.
MABL-1046
Application
IHC (P), WB, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
8G6
From
Recombinant Antibody
Specificity
This antibody binds an epitope called ANK-1 present on most NCAM isoforms expressed on a subset of NK cells of mouse and human. This epitope is selectively expressed on pre-activated, adherent natural killer NK cells (pre-A-NK cells), which are interleukin (IL)-2-stimulated NK cells, that are selectively endowed with the increased expression of integrins and ability to adhere to solid surfaces, migrate into, infiltrate, and destroy cancerous tissues.
Alternative Names
CD56; ANK1; NCAM1; NCAM-1; N-CAM-1; Ncam; Neural cell adhesion molecule 1
UniProt
P13595
Immunogen
The original antibody was generated by immunizing rats with purified mouse brain N-CAM.
Application Notes
This antibody was used to classify the nonactivated human peripheral blood NK cells expressing ANK-1 epitope using flow cytometry. It was reported that ANK-1 epitope was preferentially expressed by around 26% of NK cells (range 9–56%) and was exclusively CD56dim. This antibody was also capable of detecting ANK-1 epitope from crude human peripheral blood NK cell and affinity purified brain N- CAM lysates using western blot. This antibody was also used to test the ability of NK cells to respond to IL-2 by generation and expansion of A-NK cells with LAK activity, following depletion of ANK-1+ NK cells by treatment with mAb ANK-1 and complement. This antibody can also be used for the immunohistochemical staining of paraformaldehyde fixed paraffin embedded tissues of spleen, lymph nodes, tonsils, intestines, lungs, and liver tissues (PMID: 15356097).
Antibody First Published
Shen Li et al. A novel epitope of N-CAM defines precursors of human adherent NK cells. J Leukoc Biol. 2004 Dec;76(6):1187-99. PMID:15356097
Note on publication
This paper studies a subpopulation of NK cells expressing a unique epitope ANK-1 using an antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
