Cat. No.
MABL-1047
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
193-27
From
Recombinant Antibody
Specificity
This antibody recognizes human CD59 which is a glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. It regulates complement-mediated cell lysis, and is involved in lymphocyte signal transduction.
Alternative Names
1F5; 1F5 antigen; CD59 glycoprotein; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin
UniProt
P13987
Immunogen
This antibody was raised by immunizng mice with stimulated human leukocytes.
Application Notes
The antibody 193-27 is recommended for the identification and analysis of CD59 via various experimental techniques (Bellosillo et al., 2001). Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH) and thus this antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions.
Antibody First Published
Bellosillo et al Complement-mediated cell death induced by rituximab in B- cell lymphoproliferative disorders is mediated in vitro by a caspase-independent mechanism involving the generation of reactive oxygen species. Blood. 2001 Nov 1;98(9):2771-7. PMID:11675350
Note on publication
This article describes the use of the antibody 161-2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
