Cat. No.
MABL-1053
Application
IP, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-52
From
Recombinant Antibody
Specificity
This antibody is specific for rat CD6, which is largely restricted to cells of the T-lymphocyte lineage.
Alternative Names
OX52; MRC OX-52; MRC OX 52
UniProt
Q812A4
Immunogen
This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells.
Application Notes
This antibody recognizes rat CD6, which is largely restricted to cells of the T- lymphocyte lineage, and, as a result, this antibody can be used to identify such cells in tissues or cell suspensions (Robinson, 1986). In cell suspensions prepared from lymph node, TDL, thymus and spleen, exclusively T cells are labelled. Using the immunoperoxidase technique on cryostat tissue sections, staining is mainly confined to the T-cell areas of the spleen, lymph nodes and Peyer's patches, as well as all thymocytes. However, occassional positive cells have been observed in the B cell areas of lymphoid organs (Robinson, 1986), and consequently this antibody is used in conjunction with other markers, such as OX-19, to identify T cells.
Antibody First Published
Robinson et al. MRC OX-52: A rat T-cell antigen Volume 57, Issue 4, 1986, Pages 527-531 PMID:2937721
Note on publication
Describes the original use of this antibody in preliminary investigations into the nature of its antigen (CD6), and investigates the usefulness of this antibody as a T-cell marker.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
