Cat. No.
MABL-1687
Application
NTRL, FC
Isotype
Engineer antibody
Species Reactivity
KSHV
Clone No.
YC15
From
Recombinant Antibody
Specificity
This mAb binds KSHV glycoprotein H. KSHV is a human herpesvirus which is associated with Kaposi's sarcoma, however is also implicated in 2 distinct B cell lymphoproliferative disorders.
Alternative Names
KSHV gH; Glycoprotein H; Kaposi's sarcoma-associated herpesvirus glycoprotein H; KSHV glycoprotein H; KSHV envelope glycoprotein H; Envelope glycoprotein H
UniProt
F5HAK9
Immunogen
Antibodies were produced from a murine hybridoma.
Application Notes
YC15 was reformatted as a scFv fragment and combined with Pseudomonas aeruginosa exotoxin A to create an immunotoxin against KSHV-infected cells (Cai & Berger, 2011). YC15 was shown to bind cells expressing gH on the surface using flow cytometry, where binding was sufficient to neutralise gH activity and also induce a cytotoxic effect.
Antibody First Published
Cai & Berger. An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection. Antiviral Res. 2011 Jun;90(3)：143-50. PMID：21440007
Note on publication
Describes the design, expression and characterisation of an immunotoxin against KSHV-infected cells. YC15 was used within this study, however the details of its generation are unpublished.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
