Cat. No.
MABL-3116
Application
in vitro, in vivo, Transcytosis, ELISA
Isotype
Engineer antibody
Species Reactivity
Dog, Rat, Human, Mouse
Clone No.
FC5
From
Recombinant Antibody
Specificity
This antibody is specific for human cerebral endothelial cells (HCEC), targeting TMEM30A as its putative receptor. It cross-reacts with mouse, rat, and dog endothelial cells.
Alternative Names
TMEM-30A; Cdc50a; putative TMEM30A; HCEC; human cerebromicrovascular endothelial cells
UniProt
Immunogen
The original antibody was selected from a llama-derived nanobody phage display library by phenotypic screening against human brain endothelial cells.
Application Notes
The original antibody (llama VHH) bound specifically to HCEC, as demonstrated by an ELISA, and showed selective binding to brain endothelial cells (HCEC and HPEC). It localized to vesicular and membrane fractions, was internalized via an energy-dependent pathway without observed toxicity, and exhibited uptake into brain endothelial cells. A fusion protein version of this antibody (FC5-c-Myc-His5) demonstrated effective transmigration across an in vitro blood-brain barrier (BBB) model using HCEC monolayers, comparable to empty membranes, without altering BBB integrity. In vivo, the antibody accumulated in brain tissue after intravenous injection (Muruganandam et al., 2002; PMID: 11772942). This antibody's ability to facilitate transcytosis across the BBB via receptor-mediated pathways was demonstrated through significantly enhanced brain and cerebrospinal fluid (CSF) exposure in rat and dog in vivo models. Compared to controls, it achieved superior CNS penetration via a combination of receptor-mediated transcytosis and bulk flow. Notably, these studies revealed that the antibody is not subject to efflux by BBB transporters, enabling selective delivery without significant nonspecific uptake (Haqqani et al., 2012; PMID: 23150993).
Antibody First Published
Muruganandam et al., Selection of phage-displayed llama single-domain antibodies that transmigrate across human blood-brain barrier endothelium. FASEB J. 2002 Feb;16(2):240-2. doi: 10.1096/fj.01-0343fje. PMID:11772942
Note on publication
The original publication describes the selection and characterization of llama single-domain antibodies (sdAbs) capable of selectively binding to human cerebromicrovascular endothelial cells and transmigrating across the blood-brain barrier.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
