Key features and details | |
Cat. No. | MABL-1239 |
Name | Anti-Collagen type II mAbs |
Clone No. | AFD- CIIC1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vitro, in vivo, WB, ELISA, IHC |
Species Reactivity | Bovine, chick, Rat, Human, Mouse |
Basic Information | |
Specificity | The antibody binds the triple helical C1 epitope (GARGLT) of collagen type II. |
Alternative Name | CII; Alpha-1 type II collagen; Collagen alpha-1(II) chain |
UniProt | P02458 |
Immunogen | The original antibody was generated by immunizing DBA/1 mice with chick sternal type II collagen. |
Application Notes | This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). |
Antibody First Published | Holmdahl et al. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum. 1986 Mar;29(3):400-10. PMID:2421741 |
Note on publication | The paper describes the generation and characterization of twenty monoclonal antibodies reactive with type II collagen. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
