Anti-hINSR mAbs

Cat. No.

MABL-1824

Name

Anti-hINSR mAbs

Clone No.

AFD- IR 18-34

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, IP, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody recognises the extracellular   domain of human INSR. It does not cross-react with rat, mouse or rabbit   insulin receptors, and displays very low cross-reactivity with bovine,   porcine and ovine INSR.

Alternative Name

CD220; human insulin receptor; insulin   receptor; HHF5; IR; 18 34

UniProt

P06213

Immunogen

This antibody was raised by immunising BALB/c   mice with purified human placental insulin receptor.

Application Notes

This antibody has been used in immunoprecipitation assays, with both radiolabelled human insulin receptor   (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986). This   antibody has also been used in immunoblot analysis with purified hINSR (Soos   et al, 1986) and immunohistochemistry. This antibody has been shown to have   limited inhibitory effect on insulin binding to IM-9 cells (Soos et al,   1986). However, this antibody has been shown to stimulate insulin receptor autophosphorylation and kinase activity towards exogenous substrates (O'Brien   et al, 1987).

Antibody First   Published

Soos et al. Monoclonal antibodies reacting   with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1;  235(1)： 199–208. PMID：2427071

Note on publication

Describes the original generation of this antibody, and the characterisation of its specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.