Key features and details | |
Cat. No. | MABL-2499 |
Name | Anti-Norovirus GII.10 mAbs |
Clone No. | AFD- 5B18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, WB, ELISA, IF |
Species Reactivity | Norovirus |
Basic Information | |
Specificity | The antibody binds to a face of the GII.10 P1 subdomain close to the S domain and not openly exposed at the VLP surface (residues Val433, Glu496, Asn530, Tyr533, Thr534, and Leu535). The antibody cross-reacts with three GII genotypes GII.4, GII.10, and GII.12 but not with GI genotypes. |
Alternative Name | Capsid protein |
UniProt | Q5F4T5 |
Immunogen | The antibody was generated from a mouse immunized with GII.4 norovirus-445 VLPs. |
Application Notes | The specificity of the IgG format of this antibody was confirmed by ELISA analysis; the antibody detected intact GII.10 VLPs at different pH values with nearly identical efficacy. The structure of the Fab fragment in complex with GII.10 P domain was determined. The Western blotting results showed that the antibody IgG could detect the three GII genotypes (Hansman et al., 2012; PMID: 22278249). The antibody was used for immunofluorescence microscopy to examine VLP binding to fresh biopsies of human ileum and it bound specifically to VLPs (Murakami et al., 2013; PMID: 23799113). |
Antibody First Published | Hansman et al. Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle. J Virol. 2012 Apr; 86(7): 3635–3646. PMID:22278249 |
Note on publication | The paper describes the crystal structure and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
