| CAT.NO | MBA-0063 |
| NAME | Recombinant Anti-Polysialic antibody |
| Isotype and Format | Mouse IgG2a, Kappa |
| Clone Number | 735 |
| Alternative Name(s) of Target | PSA; N-CAM; neural cell adhesion molecule; polySA |
| UniProt Accession Number of Target Protein |
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| Published Application(s) | immunoblot, IP, WB, FC, IHC |
| Published Species Reactivity | Rat, Human, Mouse |
| Immunogen | Homopolymers of α2,8-linked sialic acid of Escherichia coli K1. |
| Specificity | Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1. |
| Application Notes | Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006). |
| Antibody First Published in | Bitter-Suermann & Roth Monoclonal antibodies to polysialic acid reveal epitope sharing between invasive pathogenic bacteria, differentiating cells and tumor cells. Immunol Res. 1987;6(4):225-37. PMID:2448401 |
| Note on publication | Describes the generation of this antibody. |
| Size | 200 μg Purified antibody. Purification |
| Purification | Protein A affinity purified |
| Supplied In | PBS with 0.02% Proclin 300 |
| Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
| Concentration | 1 mg/ml. |
