Anti-Tudor and KH domain-containing protein mAbs

Cat. No.

MABL-3154

Name

Anti-Tudor and KH domain-containing  protein mAbs

Clone No.

AFD- anti-TDRKH-RAB-C143

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human Tudor and KH domain-containing protein.

Alternative Name

TDRKH; TDRD2; Tudor domain-containing   protein 2

UniProt

Q9Y2W6

Immunogen

The original antibody was raised by panning   against a phage-display library using recombinant TDRD2 eTudor.

Application Notes

The original antibody was used for an ELISA on recombinant TDRD2 eTudor. The structure of the original antibody was determined using x-ray crystallography. This antibody was used for a pull-down assay on   TDRD2 eTudor domain (Chen et al., 2020; PMID:31288074). This antibody was   used for immunofluorescence on different human cell lines. This antibody showed staining of the cytoplasm for all cell lines tested (Hornsby et al.,   2015; PMID:26290498).

Antibody First   Published

Hornsby et al. A High Through-put Platform   for Recombinant Antibodies to Folded Proteins Mol Cell Proteomics. 2015   Oct;14(10):2833-47. PMID:26290498

Note on publication

The original paper describes an   industrialized platform to generate antigens and validated recombinant antibodies for 346 transcription factors (TFs) and 211 epigenetic antigens.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.